Composite

Part:BBa_K4164013:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-09-30)


Inductive expression of BSS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1600
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1001
    Illegal NgoMIV site found at 1052
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We recombined BBa_K4164001 to plasmid pET-29a+ by homologous recombination.


Source

This sequence is composed of BBa_J64997, BBa_K4164995, BBa_K2556021, BBa_K416001and BBa_K923004.

References