Composite
Part:BBa_K4164013:Design
Designed by: Bo Cheng Group: iGEM22_NAU-CHINA (2022-09-30)
Inductive expression of BSS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1600
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1001
Illegal NgoMIV site found at 1052 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We recombined BBa_K4164001 to plasmid pET-29a+ by homologous recombination.
Source
This sequence is composed of BBa_J64997, BBa_K4164995, BBa_K2556021, BBa_K416001and BBa_K923004.